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. 2014 Dec 11;10(12):e1004866. doi: 10.1371/journal.pgen.1004866

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© 2014 Warburton-Pitt et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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In amphid channel and phasmid cilia, NPHP-2::GFP, ARL-13::GFP, and GFP::UNC-119 signals overlap with GT335 staining. ARL-13::GFP and GT335 staining overlap completely. NPHP-2::GFP does not overlap completely with GT335. TZ staining by GT335 is frequently visible, as in the NPHP-2::GFP overlay. Though the signal of GFP::UNC-119 is dim due to the staining procedure, GFP::UNC-119 appears not to overlap completely with GT335. Data is quantified in S6A Figure.