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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Exp Biol Med (Maywood). 2014 May 28;239(10):1380–1389. doi: 10.1177/1535370214532759

Figure 2.

Figure 2

Effects of GEN-supplementation on markers of resorption (a) RANKL:OPG ratio and (b) RANKL protein expression in vertebral bone of PF-, EtOH-, PF/GEN- and EtOH/GEN- treated mice (n=5/group). Gene expression of RANKL and OPG were determined by real-time PCR as described in the Materials and Methods. RANKL protein expression was assessed by Western blot as described in the Materials and Methods. Data is expressed as mean ± SEM. Statistical significance was determined by a two-tailed Student’s T-test. Values with different letters are significantly different from each other (P < 0.05).