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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Exp Biol Med (Maywood). 2014 May 28;239(10):1380–1389. doi: 10.1177/1535370214532759

Figure 4.

Figure 4

The effects of GEN on markers of osteoblastogenesis, bone marrow cells were isolated from 6wk female C57Bl6 mice (n=3), plated in quadruplicate, and grown in osteogenic media supplemented with GEN for 10d followed by (a) alkaline phosphatase staining. Gene expression of (b) RANKL, (c) Osteoprogetrin (OPG), and (d) osterix in bone marrow cultures grown in osteogenic media supplemented with GEN or E2 for 10 days. For each treatment group, cells were plated in quadruplicate. Data are expressed as mean ± S.E.M. Statistical significance for alkaline phosphastase staining was determined by a two-tailed Student’s T-Test, *p<0.05 control vs. GEN, **p< 0.05 GEN vs. E2.