Table 1. Activity of Recombinant Yeast Microsomal P450s in Catalyzing Epoxide Formation from Compound 1a.
| activity, % product conversion |
|||
|---|---|---|---|
| system | SOD/catalase | epoxide (2) | 21-hydroxy (3) |
| CYP17A1-WT | – | 29.6 ± 1.8 | 29.6 ± 0.8 |
| + | 29.5 ± 1.5 | 29.5 ± 1.5 | |
| CYP17A1-A105L | – | 14.0 ± 0.5 | 70.4 ± 1.3 |
| + | 14.3 ± 0.6 | 73.7 ± 1.3 | |
| CYP21A2-WT | – | 0.1 ± 0.0 | 3.6 ± 0.2 |
| + | 0.1 ± 0.0 | 4.3 ± 0.1 | |
| CYP21A2-V359A | – | 0.2 ± 0.0 | 3.4 ± 0.3 |
| + | 0.3 ± 0.1 | 2.9 ± 0.2 | |
a
All data are expressed as percent conversion of compound 1 to epoxide 2 or 21-hydroxy product 3 by recombinant yeast microsomes containing specified P450 and POR (60–200 μg of protein, 10–30 pmol of P450) with or without SOD and catalase after 40 min. Data represent the mean ± standard deviation for three experiments.