Table 2. Epoxidation and Hydroxylation of Steroids by Purified Human P450s and PORa.
| compound 1 |
progesterone |
||||
|---|---|---|---|---|---|
| P450 | epoxide (2) | 21-hydroxy (3) | 16α-hydroxy | 17-hydroxy | 21-hydroxy |
| CYP17A1-WT | 41.0 ± 1.0 | 41.5 ± 1.4 | 21.7 ± 0.1 | 66.9 ± 1.4 | 3.0 ± 0.2 |
| CYP17A1-A105L | 16.2 ± 0.7 | 81.6 ± 1.2 | 6.6 ± 0.1 | 85.6 ± 0.5 | 6.5 ± 0.1 |
| CYP21A2-WT | 0.07 ± 0.02 | 41.5 ± 2.5 | 0.27 ± 0.04 | ND | 54.9 ± 1.9 |
| CYP21A2-V359A | 0.25 ± 0.01 | 43.5 ± 0.7 | 8.46 ± 0.64 | ND | 11.8 ± 0.6 |
a
All data are expressed as percent conversion of substrate (20 μM) to products by reconstituted system containing purified P450 (20–30 pmol), POR (80–120 pmol), and CYMS lipid (20 μg of protein) after 30 min. Data represent the mean ± standard deviation for three experiments; ND, not detectable.