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| enzyme | Km (mM) | kcat (s–1) | kcat/Km (M–1 s–1) | Ki, d-serine (mM) | Ki, l-cysteine (μM) |
|---|---|---|---|---|---|
| wild type | 2.0 ± 0.1 | 330 ± 3 | (16.5 ± 0.8) × 104 | 7 | 55 |
| C458Aa | 6.9 ± 0.3 | 512 ± 10 | (7.4 ± 0.4) × 104 | – | – |
| H61A | nmab | nmab | nmab | – | – |
| H19A | 24.4 ± 1.7 | 31 ± 1 | (0.13 ± 0.01) × 104 | 8 | 1500 |
| H134A/N136A | 33.2 ± 2 | 112 ± 3 | (0.34 ± 0.02) × 104 | 5.5 | 185 |
From ref (14).
No measurable activity.
