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. 2014 Oct 22;53(48):7459–7470. doi: 10.1021/bi501025s

Figure 3.

Figure 3

EMS studies of binding of IHF to minimal duplex substrates. (A) Representative polyacrylamide gel showing that IHF binds to the minimal 27 bp I1-specific substrate to afford a distinct retarded complex. We note that upward “smearing” of the retarded band is observed at IHF concentrations of >100 nM (not shown). (B) Representative polyacrylamide gel showing that IHF binds to the minimal 27 bp I2 substrate to afford a smear on the gel. (C) Representative polyacrylamide gel showing that IHF binds to the 75 bp [R3-I1-R2] duplex substrate to afford a distinct retarded complex. (D) Representative polyacrylamide gel showing that IHF binds to the 75 bp [I2-R3-I1] duplex substrate to afford a distinct retarded complex. The positions of free and bound DNA complexes are indicated at the right of each gel image.