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. 2014 Oct 22;53(48):7459–7470. doi: 10.1021/bi501025s

Figure 4.

Figure 4

Quantitative analysis of EMS binding data. The EMS data (representative data presented in Figures 2 and 3) were converted to fraction bound DNA versus IHF concentration as described in Experimental Procedures. (A) Ensemble of EMS data for binding of IHF to the cos274 (blue), [R3-I1-R2] (red), and [I2-R3-I1] (green) duplexes. Each data point is the average of at least three separate experiments with the standard deviation indicated with error bars. The ensemble of data was simultaneously analyzed according to the nonspecific finite lattice DNA binding model as described in Experimental Procedures. The solid lines represent the best fits of the data, and the derived binding parameters are presented in Table 1. (B) EMS data for binding of IHF to the minimal I1-specific duplex. Each data point is the average of at least three separate experiments with the standard deviation indicated with error bars. The data were analyzed according to the competitive specific/nonspecific finite lattice DNA binding model as described in Experimental Procedures. The solid line represents the best fit of the data, and the derived binding parameters are presented in Table 1.