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Second-order rates of acylation of Bocillin-FL against full-length and truncated constructs of wild-type PBP2 and PBP2 from the penicillin-resistant strain 6140. For each experiment, the pseudo-first-order rates of acylation (ka) were plotted vs the concentration of Bocillin-FL. Shown is the average plot of at least two or more independent experiments, where the slope of the line yields the second-order k2/Ks value.
