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. 2014 Dec 11;9(12):e106033. doi: 10.1371/journal.pone.0106033

Figure 5. Functional analysis of serial deletions of PeMADS4 promoter.

Figure 5

(A) Serial deletion constructs of PeMADS4 promoter. (B–Q) Histochemical assay of flower organs bombarded with serial deletions of PeMADS4 promoter shown in the order of pBI-Pe4p-2313 (B–E), pBI-Pe4p-1497 (F–I), pBI-Pe4p-935 (J–M) and pBI-Pe4p-375 (N–Q). Constructs were bombarded into four independent floral buds, and results are representative of three independent bombardment experiments. Scale bar  = 0.5 mm. (R) Dual luciferase assay of the serial deletions of PeMADS4 promoter. The same letters above the bars are not statistically different by T-test analysis (p<0.01). Data are mean ± SD (n = 6). All constructs were analyzed for promoter activities of driving luciferase expression by bombardment into two floral buds, and results are representative of three independent bombardment experiments.