Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 15;25(25):4063–4071. doi: 10.1091/mbc.E14-06-1153

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Cannet et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — A siRNA screening approach identifies Trio as a potential GEF of Rac1 in dividing cells. HeLa cells were transfected with MgcRacGAP siRNA (siMgcRacGAP) in combination with control siRNA, Rac1 siRNA, or siRNAs targeting the indicated GEFs (SMART pools) and scored for the presence of multinucleated cells 48 h posttransfection. (A) Immunofluorescence of HeLa cells stained with DAPI (nuclei, red) and α-tubulin (green). Asterisks, multinucleated cells. Scale bar, 20 μm. (B) Quantification of the percentage of multinucleated cells. Five hundred cells were counted per experiment. Three independent experiments. Mean ± SEM. *p < 0.02.