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. 2014 Dec 15;25(25):4083–4095. doi: 10.1091/mbc.E14-03-0832

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© 2014 He, Yam, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — ER-associated lipid droplets in cds1-9 S. pombe cells grow by expansion. (A) A time-lapse sequence showing emergence and expansion of the Tgl4-GFP–marked lipid droplet in cds1-9 mutant cell (indicated by yellow arrowhead) at the restrictive temperature of 36°C. Also shown are the corresponding phase-contrast images. (B) A time-lapse sequence of GFP-ADEL– expressing cds1-9 mutant cell documents emergence of the ER-associated LD at the restrictive temperature of 36°C. Insets, magnified views of an indicated area. (A, B) Cells grown in rich yeast extract–based medium. Time is in hours and minutes. Scale bar, 5 μm; inset, 2 μm.