TABLE 3:

Analysis of skilled locomotion.

Parameter	wt	Pax7-Cre/cKO
Identical rungs targeted by FPs and HPs (%)a	95.6 ± 0.2	89.5 ± 2.1
Functional paw placement (%)b	97.0 ± 0.1	96.2 ± 0.2
Distance between HPs (cm/g)c	1.3 ± 0.1	1.9 ± 0.1***

Mice were recorded while walking on a horizontal ladder (see Supplemental Figure S6D). The wt mice crossed the ladder without slipping and by placing the HP on the same rung that previously had been occupied by their ipsilateral forepaw (FP), whereas mutant mice showed a slightly less effective targeting of identical rungs accompanied by several paw slips. Measurements done for each step were averaged. Mice examined: wt, 27; Pax7-Cre/cKO, 20. Mean ± SEM.

^aFL-HL coordination was assessed by calculating the percentage of identical rungs targeted by ipsilateral FPs and HPs.

^bPrecise placements of FPs on rungs were analyzed, and percentage of paw misplacements was calculated.

^cThe larger distance between HPs suggested loss of HL strength.

***P < 0.001, unpaired Student's t test.