TABLE 3:
Dictyostelium strains used in this study.
| Strain | Genotype | Experimental applications |
|---|---|---|
| WT::GFP-3xAsp | KAX3::pBIG:GFP-3xAsp | Suspension |
| WT::GFP-myoII | KAX3::pBIG:GFP-myoII | Suspension |
| myoII null | mhcA(HS1) null | Suspension, cytokinesis |
| WT::GFP-3xAsp; mCh-tub | KAX3::pBIG:GFP-3xAsp; pDRH:mCh-tub | 3xAsp localization, MPA, suspension |
| myoII::GFP-3xAsp; mCh-myoII | mhcA(HS1)::pBIG:GFP-3xAsp; pDRH:mCh-myoII | 3xAsp/myoII localization, MPA, suspension |
| myoII::GFP-myoII; mCh-myoII | mhcA(HS1)::pBIG:GFP-myoII; pDRH:mCh-myoII | myoII localization, suspension |
| WT::GFP-3xAsp | Ax3(Rep orf+)::pDRH:GFP-3xAsp | cDNA library suspension selection, recapitulations |
| WT::GFP-3xAsp control | Ax3(Rep orf+)::pDRH:GFP-3xAsp; pLD1A15SN | Suspension |
| myoII::GFP-3xAsp | mhcA(HS1)::pDRH:GFP-3xAsp | 3xAsp localization |
| WT::GFP-myoII | Ax3(Rep orf+)::pDRH:GFP-myoII; pLD1A15SN | Suspension |
| WT control | Ax3(Rep orf+)::pLD1A15SN | Suspension, DAPI, cortical tension, cytokinesis |
| WT::rmd1hp | Ax3(Rep orf+)::pLD1A15SN:rmd1hp | DAPI, cortical tension, cytokinesis |
| myoII::GFP-3xAsp; suppressor | mhcA(HS1)::pDRH:GFP-3xAsp; pLD1A15SN:suppressor | 3xAsp localization, suspension |
| myoII::GFP-3xAsp control | mhcA(HS1)::pDRH:GFP-3xAsp; pLD1A15SN | 3xAsp localization, TIRF, suspension, cytokinesis |
| myoII::mCh-3xAsp; GFP-RMD1 | mhcA(HS1)::pDRH:mCh-3xAsp; pDM181:GFP-RMD1 | 3xAsp localization |
| myoII::mCh-3xAsp; GFP-mmsdh | mhcA(HS1)::pDRH:mCh-3xAsp; pDM181:GFP-mmsdh | 3xAsp localization |
| myoII::mCh-3xAsp control | mhcA(HS1)::pDRH:mCh-3xAsp; pDM181:GFP | 3xAsp localization |
| myoII::GFP-3xAsp; rmd1hp | mhcA(HS1)::pDRH:GFP-3xAsp; pLD1A15SN:rmd1hp | 3xAsp localization |
| WT::GFP-RMD1; RFP-tub | Ax3(Rep orf+)::pDM181:GFP-RMD1; pDRH:RFP-tub | Protein localization |
| WT::GFP-RMD1 | Ax3(Rep orf+)::pDM181:GFP-RMD1 | Protein localization, agar overlay |
| WT::GFP-mmsdh | Ax3(Rep orf+)::pDM181:GFP-mmsdh | Protein localization, agar overlay |
| WT::GFP control | Ax3(Rep orf+)::pDM181:GFP | Protein localization, agar overlay |
| myoII::GFP-RMD1 | mhcA(HS1)::pDM181:GFP-RMD1 | Protein localization, agar overlay |
| myoII::GFP-mmsdh | mhcA(HS1)::pDM181:GFP-mmsdh | Protein localization, agar overlay |
| myolI::GFP control | mhcA(HS1)::pDM181:GFP | Protein localization, agar overlay |
| WT::mCh-rcdBB; cnx-GFP | Ax3(Rep orf+)::pDRH:mCh-rcdBB; pDEXRH:cnx-GFP | Protein localization |
| WT::RMD1-GFP | Ax3(Rep orf+)::pDM181:RMD1-GFP | Protein localization |
| myoII::GFP-3xAsp, RMD1 | mhcA(HS1)::pDRH:GFP-3xAsp; pLD1A15SN:RMD1 | TIRF |
| myoII::GFP-3xAsp, mmsdh | mhcA(HS1)::pDRH:GFP-3xAsp; pLD1A15SN:mmsdh | TIRF |
| myoII::GFP-3xAsp, actin | mhcA(HS1)::pDRH:GFP-3xAsp; pLD1A15SN:actin | TIRF |
| myoII::GFP-3xAsp, mCh-RMD1 | mhcA(HS1)::pBIG:GFP-3xAsp; pDRH:mCh-RMD1 | TIRF, agar overlay, cytokinesis |
| myoII::GFP-3xAsp, mCh-mmsdh | mhcA(HS1)::pBIG:GFP-3xAsp; pDRH:mCh-mmsdh | TIRF, agar overlay |
| myoII::GFP-3xAsp, mCh control | mhcA(HS1)::pBIG:GFP-3xAsp; pDRH:mCh | TIRF, agar overlay, cytokinesis |
| myoII::GFP-myoII, pLD1 | mhcA(HS1)::pDRH:GFP-myoII; pLD1A15SN | WT myosin II furrow accumulation, agar overlay |
| myoII::GFP-myoII, rmd1hp | mhcA(HS1)::pDRH:GFP-myoII; pLD1A15SN:rmd1hp | WT myosin II furrow accumulation, agar overlay |
| myoII::GFP-2xELC-3xAsp | mhcA(HS1)::pDM181:GFP-2xELC-3xAsp | Protein localization, TIRF, suspension, cytokinesis |