Figure 1. The MSL-complex is redistributed in roX mutants.

(A) MSL3 immunostaining on polytene chromosomes from wild type, mle, mof and roX mutant males. Note that MSL3 only targets a subset of sites on the X-chromosome, the 4^th chromosome and the chromocenter (indicated by the arrow) in roX mutants. (B) DNA-FISH with a probe against the 1.686 g/cm³ satellite repeat from pericentromeric regions of chromosomes 2 and 3 (1686 probe) combined with MSL3 immunostaining, on interphase nuclei of brain cells from third instar male larvae wild type, mof and roX mutants. (C) Percentage colocalization of 1686 probe signal and MSL3 staining from 8 replicates each of the genotypes: wild type, mof and roX (30–50 nuclei scored per replicate). The bars indicate the mean colocalization and the whiskers indicate standard deviations. Significant differences are indicated by *** (Independent two-sample t-test, p<0.001). (D) DNA-FISH with the 1686 probe combined with MSL3 immunostaining on metaphase nuclei of brain cells from third instar larvae wild type and roX mutant males. Note that on metaphase chromosomes MSL3 colocalization with centromeres is not detected in roX mutants.