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. 2014 Dec 11;10(12):e1004865. doi: 10.1371/journal.pgen.1004865

Figure 4. In roX mutants Hoppel is enriched in MSL-complex.

Figure 4

(A) Sequence motif enriched in MSL-bound regions on heterochromatin of roX mutants. (B) MOF and MSL1 ChIP-seq enrichment profiles, with a 2000 bp smoothing, for the representative chromosome arm 3L from wild type and roX mutant males. Numbers along the x-axis denote chromosomal positions along the chromosome in Mb. The y-axis shows the ChIP enrichment over input as log2 ratios. The mapping of the motif along 3L is indicated. Below the motif track the ratio roX-MOF/WT-MOF is plotted. Note that in roX mutants MSL is enriched in the pericentromeric region (indicated by a yellow box). (C) DNA-FISH with a 33 nucleotide long probe against the heterochromatic motif shown in A combined with MSL1 immunostaining, on polytene chromosomes of roX mutant males. Note the overall colocalization between the MSL1 bound regions and the motif hybridisation.