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. 2014 Dec 11;9(12):e114791. doi: 10.1371/journal.pone.0114791

Figure 4. Time-course and dose-dependent effects of LIGHT on bronchial epithelial (BEAS-2B) cells.

Figure 4

(A) BEAS-2B cells were stimulated with 50 ng/ml LIGHT and examined for the time-course effect on expression of mRNA for each of IL-8, IL-6, MCP-1 and RANTES. LIGHT significantly induced mRNA for each of IL-6, IL-8 and MCP-1. n = 4 separate experiments. *: p<0.05, **: p<0.01 vs 0 h. (B) BEAS-2B cells were stimulated with various concentrations of LIGHT (0, 1, 10, 100 ng/ml) for 1 h and evaluated for expression of mRNA for each of IL-8 and IL-6. LIGHT induced IL-8 and IL-6 mRNA dose-dependently. n = 4 separate experiments. ** and ††: p<0.01 vs 0 ng/ml. (C) BEAS-2B cells were stimulated with 100 ng/ml LIGHT for 24 h, and the protein concentrations in the cell supernatants were determined by ELISA. LIGHT significantly induced IL-8 and IL-6 proteins. n = 6 separate experiments. **: p<0.01.