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. 2014 Dec 11;9(12):e114791. doi: 10.1371/journal.pone.0114791

Figure 6. LTβR signaling in bronchial epithelial cells induced by LIGHT.

Figure 6

BEAS-2B cells were stimulated with 50 ng/ml LIGHT, and cell lysates were prepared 0, 5, 15, 20, 30 and 90 minutes later to evaluate phosphorylation of Erk, JNK, p38 and IκB. The MAPKs were phosphorylated for up to 15 minutes, and their signaling was activated. IκB was also phosphorylated at the same time and induced NF-κB release and translocation to nucleus. (A) BEAS-2B cells were pre-treated for 1 h with various inhibitors of phosphorylation of MAPKs. U0126 significantly inhibited IL-8 production by the cells. (B) BEAS-2B cells were pre-treated for 1 h with BAY11-7082. BAY11-7082 significantly inhibited IL-8 production. (C) We performed the same experiments using NHBE cells, which are primary normal human bronchial epithelial cells. LTβR siRNA, U0126 and BAY11-7082 significantly inhibited IL-8 production by NHBE cells in the same manner as seen for BEAS-2B cells.