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. 2014 Dec 11;9(12):e114791. doi: 10.1371/journal.pone.0114791

Figure 7. Erk1/2 signaling and NF-κB release.

Figure 7

(A) To evaluate the relationship between Erk1/2 signaling and NF-κB release, BEAS-2B cells were pretreated with U0126 (10 µM) 1 h before stimulation with LIGHT. U0126 did not inhibit IκBα phosphorylation or NF-κB translocation from the cytoplasm to the nucleus. (B) We evaluated the effect of LTβR knockdown on Erk1/2 signaling and NF-κB release. BEAS-2B cells were transfected with negative control siRNA (NC siRNA) or LTβR siRNA#2, stimulated with LIGHT (50 ng/ml) and analyzed by western blotting. The cells that were transfected with LTβR siRNA showed attenuation of Erk1/2 phosphorylation and NF-κB translocation induced by LIGHT.