Figure 8. Effect of LTβR signaling on transcription factor-driven luciferase activity by transient transfection of BEAS-2B cells.

BEAS-2B cells were transfected with a plasmid having luciferase as a reporter gene, controlled by a synthetic promoter containing NF-κB, AP-1 or serum response element (SRE). After 24 h, the cells were stimulated with LIGHT (50 ng/ml) or TNF-α (10 ng/ml) as a positive control, with/without U0126. The data are the mean ± SEM of four independent experiments performed in triplicate. Significant differences (p<0.01) are indicated with **.