Figure 4.

Overexpression of sypK activates qrr1 expression. Levels of qrr1 expression in the reporter strain TIM303 harboring vector (pTM214), P_trc-sypK (pTM367), or P_trc-sypL (pTM368) grown ±100 μmol/L IPTG. The wild-type strain ES114 harboring pTM214 was used as the GFP-negative control for quantifying GFP levels. Graphical and error bars represent the averages and standard deviations of triplicate biological replicates, respectively. Comparisons with significance based on two-way ANOVA with Tukey's multiple comparisons are shown with *(P-value <0.01). Comparisons between pTM367-harboring strains with strains harboring either pTM214 or pTM368 are significant (P-value <0.001). Experiment was performed three times, with similar results.