Figure 5.

Overexpression of sypK enhances qrr1-dependent motility. (A) Soft-agar motility migration distances by WT (ES114; closed symbols) and Δqrr1 (TIM305; open symbols) harboring vector (pTM214; circles) or P_trc-sypK (pTM367; squares). TBSW motility plates contained chloramphenicol and 100 μmol/L IPTG. Points and error bars (too small to visualize) represent means and standard deviations of spot diameter (in mm) for quadruplicate biological replicates, respectively. Experiment was performed twice, with similar results. (B) Image of motility plate used in (A) at 4 h.