Table 1.
Vibrio fischeri strains and plasmids used in this study
| Strain | Genotype | References |
|---|---|---|
| ES114 | Wild-type V. fischeri | Boettcher and Ruby (1990); Ruby et al. (2005); Mandel et al. (2008) |
| CL59 | luxO::luxOD47E | Lupp and Ruby (2005) |
| DRO1B3 | sypJ::Tn5 | This study |
| DRO216 | luxO::luxOD47E attTn7::Pqrr1-gfp erm | This study |
| DRO222 | sypI::Tn5 [NT] | This study |
| DRO5F11 | sypI::Tn5 | This study |
| EVS102 | ΔluxCDABEG | Bose et al. (2008) |
| KV4829 | ΔluxU | This study |
| KV5069 | ΔsypL | Shibata et al. (2012) |
| KV5972 | ΔluxQ | This study |
| KV6010 | ΔluxP | This study |
| KV6529 | ΔluxQ attTn7::Pqrr1-gfp erm | This study |
| KV6530 | ΔluxU attTn7::Pqrr1-gfp erm | This study |
| KV6549 | ΔluxP attTn7::Pqrr1-gfp erm | This study |
| KV6629 | ΔsypL attTn7::Pqrr1-gfp erm | This study |
| TIM303 | attTn7::Pqrr1-gfp erm | This study |
| TIM305 | Δqrr1 | Miyashiro et al. (2010) |
| TIM306 | ΔluxO | Miyashiro et al. (2010) |
| TIM311 | ΔluxO attTn7::Pqrr1-gfp erm | This study |
| TIM358 | ΔlitR | Miyashiro et al. (2010) |
| TIM374 | ΔluxPQ attTn7::Pqrr1-gfp erm | This study |
| TIM394 | ΔsypK | This study |
| TIM395 | ΔsypK attTn7::Pqrr1-gfp erm | This study |
| Plasmid | Description | References |
|---|---|---|
| pCLD56 | pKV282 sypG | Morris and Visick (2013) |
| pEVS107 | R6Kori oriT mini-Tn7 mob erm kan | McCann et al. (2003) |
| pEVS79 | pBC SK (+) oriT cat | Stabb and Ruby (2002) |
| pKV282 | Mobilizable vector; TetR | Morris et al. (2011) |
| pLosTfoX | pEVS79 tfoX | Pollack-Berti et al. (2010) |
| pTM146 | ColE1ori bla cat kan gfp PtetA-mCherry | Miyashiro et al. (2010) |
| pTM214 | lacIqPtrc-mCherry | Miyashiro et al. (2011) |
| pTM239 | pEVS107 Pqrr1-gfp erm | This study |
| pTM268 | pVSV105 Pqrr1-gfp PtetA-mCherry | Miyashiro et al. (2010) |
| pTM327 | pEVS79 ΔluxPQ | This study |
| pTM367 | pTM214 ΔmCherry::sypK | This study |
| pTM368 | pTM214 ΔmCherry::sypL | This study |
| pTM375 | pEVS79 ΔsypK | This study |
| pVAR18 | Mobilizable suicide vector; ΔluxU | Ray and Visick (2012) |
| pVAR29 | Mobilizable suicide vector; ΔluxQ | Ray and Visick (2012) |
| pVAR30 | Mobilizable suicide vector; ΔluxP | Ray and Visick (2012) |
| pVAR48 | pVSV105 luxQ-FLAG | This study |
| pVAR50 | pVSV105 luxQA216P-FLAG | This study |
| pVAR51 | pVSV105 luxQH378A-FLAG | This study |
| pVAR70 | pKV282 sypK-FLAG | This study |
| pVSV105 | R6Kori ori(pES213) RP4 oriT cat | Dunn et al. (2006) |