Figure 1.

Defining optimal conditions for biofilm formation in vitro. Biofilm formation was assessed in the wild-type (WT) strains UAMS-1 (U1), LAC, and their isogenic sarA mutants (S) using tryptic soy broth (TSB) or TSB supplemented with salt and glucose (biofilm media, BFM) with and without first coating the substrate with human plasma. Single asterisk indicates statistical significance associated with growth conditions. Double asterisk indicates statistical significance of the sarA mutant by comparison to its isogenic parent strain under these optimized conditions.