Figure 9.

Impact of purified aureolysin in contemporary clinical isolates as a function of methicillin resistance status. A microtiter plate assay was used to assess biofilm formation under standard conditions (−) or after the addition of purified aureolysin at a concentration of 62.5 nM (+). The experiments included 30 primary clinical isolates obtained from the collection at Arkansas Children's Hospital. Asterisks indicate strains in which the addition of aureolysin had a statistically significant impact on biofilm formation. Note that this includes the preponderance of both MRSA and MSSA strains and, conversely, that both groups include a limited number of strains in which biofilm formation was unaffected by the addition of aureolysin (bars). However, these latter strains generally did not form a robust biofilm (OD < 1.0).