Table 1.
Strains and plasmids used in this study. All mutations were made in the wild-type strain JH1140. All the plasmids came from Escherichia coli DH5α cells
| Strains used | Plasmid intermediate | Description | References |
|---|---|---|---|
| S. mutans | |||
| JH1140 ATCC 55676 | Wild-type bacteriocin-producing strain | Strain (Hillman et al. 1998) | |
| lanA:IFDC2 | pIFDC2 | Gene replacement strain | Plasmid (Xie et al. 2011) |
| Δ(-40-33) | pΔ(-40-33) | 8 amino acid(AA) N-terminal truncation | This study |
| Δ(-40-28) | pΔ(-40-28) | 13 AA N-terminal truncation | This study |
| Δ(-40-24) | pΔ(-40-24) | 17 AA N-terminal truncation | This study |
| Δ(-40-37) | pΔ(-40-37) | 4 AA N-terminal truncation | This study |
| Δ(-36-33) | pΔ(-36-33) | Internal 4 AA truncation | This study |
| Δ(-32-28) | pΔ(-32-28) | Internal 4 AA truncation | This study |
| Δ(-27-24) | pΔ(-27-24) | Internal 4 AA truncation | This study |
| Δ(-23-20) | pΔ(-23-20) | Internal 4 AA truncation | This study |
| Δ(-7-2)* | pΔ(-7-2) | Internal 6 AA truncation | This study |
| F(-27)A | pF(-27)A | Single alanine substitution at presumed box | This study |
| D(-26)A | pD(-26)A | Single alanine substitution at presumed box | This study |
| V(-25)A | pV(-25)A | Single alanine substitution at presumed box | This study |
| Q(-24)A | pQ(-24)A | Single alanine substitution at presumed box | This study |
| Q(-24)InsAla | p Q(-24)InsAla) | Alanine insertion at EDLF motif | This study |
| E(-23)A | pE(-23)A | Single alanine substitution at EDLF motif | This study |
| E(-23)InsAla | p E(-23)InsAla | Alanine insertion at EDLF motif | This study |
| D(-22)A | pD(-22)A | Single alanine substitution at EDLF motif | This study |
| D(-22)InsAla | p D(-22)InsAla | Alanine insertion at EDLF motif | This study |
| L(-21)A | pL(-21)A | Single alanine substitution at EDLF motif | This study |
| Δ(-21) | pΔ(-21) | Single AA truncation at EDLF motif | This study |
| L(-21)InsAla | p L(-21)InsAla | Alanine insertion at EDLF motif | This study |
| F(-20)A | pF(-20)A | Single alanine substitution at EDLF motif | This study |
| Δ(-20) | pΔ(-20) | Single AA truncation at EDLF motif | This study |
| Δ(-19) | pΔ(-19) | Single AA truncation at EDLF motif | This study |
| AALF | pAALF | Double alanine substitution at ED | This study |
| EDED | pEDED | ED substitution for LF | This study |
| N+6xHis* | pN+6xhis | 6xHis addition at N-terminus | This study |
| N6xHis* | pN6xhis | 6xHis substitution at the N-terminus | This study |
| M. luteus ATCC 10760 | Indicator strain in the differed antagonism assay | Strain (Chen et al. 2013) | |
| E. coli DH5 α | pCR®2.1-TOPO® | Intermediate cloning host | Invitrogen® |