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. 2012 Apr 25;2(2):127–170. doi: 10.3390/bios2020127

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(a) Neuronal network derived from murine spinal cord tissue (92 days in vitro), grown on the recording matrix of a 64-electrode array plate. (Reprinted from [70]. ©2007, with permission from Elsevier) (b) Developmental changes in neuronal activity. Random bursts were observed at DIV4, tightly synchronized activity appeared at DIV11. The mature neuronal activity consisted of a complicated, high order pattern of spike-like firing and bursting at DIV45. (Reprinted from [71]. © 1996, with permission from Elsevier).