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. 2014 Dec 11;9(12):e114434. doi: 10.1371/journal.pone.0114434

Table 2. The relative expression of Bp4CL1 and Bp4CL2 genes in silver birch (Betula pendula) stems and leaves.

Gene Stems Leaves
Clone/Line Atub PP2A Atub PP2A
Bp4CL1 A 1.27±0.21 ad 1.28±0.24 a 1.77±1.17 a 1.91±1.45 a
A1 0.85±0.25 * 0.48±0.15 *** 1.27±0.94 0.94±0.66 *
A5 1.28±0.18 0.78±0.4 * 1.79±1.29 0.90±0.69 *
E5382 0.52±0.05 bc 0.38±0.22 b 1.92±0.91 ab 0.63±0.40 b
E5382/3 0.49±0.15 0.25±0.15 0.92±0.49 0.96±0.34
E5396 0.63±0.05 b 0.31±0.06 b 1.79±1.93 b 0.67±0.54 b
E5396/4 0.82±0.15 * 0.31±0.11 1.48±1.14 0.64±0.38
R 1.82±0.15 cd 0.56±0.2 b 1.75±1.54 ab 1.14±0.45 ab
Bp4CL2 A 0.88±0.22 a 0.88±0.16 a 2.92±2.85 3.12±3.38 a
A1 0.85±0.09 0.48±0.08 *** 2.25±2.37 2.41±2.18
A5 1.17±0.54 0.71±0.19 4.22±3.87 2.70±2.17
E5382 0.40±0.25 ab 0.33±0.28 b 2.34±1.78 0.55±0.29 b
E5382/3 0.13±0.09 0.06±0.04 0.50±0.31 0.51±0.14
E5396 0.29±0.09 b 0.14±0.05 b 1.50±1.39 0.33±0.10 b
E5396/4 1.05±0.57 ** 0.37±0.16 * 1.64±1.61 0.72±0.61
R 1.75±1.54 ab 1.14±0.45 ab 4.09±3.2 3.82±2.75 a

Values represent means and standard deviations calculated using Atub or PP2A gene as reference gene (n = 3–16). Statistical examination of data was performed using the independent-samples t test, Welch Two Sample t test or Wilcoxon test (with Bonferroni correction in the case of clonal differences). Different letters denote significant (P<0.05) difference between the clones. Stars denote significant (*P<0.05, **P<0.01, ***P<0.001) difference between transgenic line to non-transgenic clone.