Table 2. The relative expression of Bp4CL1 and Bp4CL2 genes in silver birch (Betula pendula) stems and leaves.
Gene | Stems | Leaves | |||||||
Clone/Line | Atub | PP2A | Atub | PP2A | |||||
Bp4CL1 | A | 1.27±0.21 | ad | 1.28±0.24 | a | 1.77±1.17 | a | 1.91±1.45 | a |
A1 | 0.85±0.25 | * | 0.48±0.15 | *** | 1.27±0.94 | 0.94±0.66 | * | ||
A5 | 1.28±0.18 | 0.78±0.4 | * | 1.79±1.29 | 0.90±0.69 | * | |||
E5382 | 0.52±0.05 | bc | 0.38±0.22 | b | 1.92±0.91 | ab | 0.63±0.40 | b | |
E5382/3 | 0.49±0.15 | 0.25±0.15 | 0.92±0.49 | 0.96±0.34 | |||||
E5396 | 0.63±0.05 | b | 0.31±0.06 | b | 1.79±1.93 | b | 0.67±0.54 | b | |
E5396/4 | 0.82±0.15 | * | 0.31±0.11 | 1.48±1.14 | 0.64±0.38 | ||||
R | 1.82±0.15 | cd | 0.56±0.2 | b | 1.75±1.54 | ab | 1.14±0.45 | ab | |
Bp4CL2 | A | 0.88±0.22 | a | 0.88±0.16 | a | 2.92±2.85 | 3.12±3.38 | a | |
A1 | 0.85±0.09 | 0.48±0.08 | *** | 2.25±2.37 | 2.41±2.18 | ||||
A5 | 1.17±0.54 | 0.71±0.19 | 4.22±3.87 | 2.70±2.17 | |||||
E5382 | 0.40±0.25 | ab | 0.33±0.28 | b | 2.34±1.78 | 0.55±0.29 | b | ||
E5382/3 | 0.13±0.09 | 0.06±0.04 | 0.50±0.31 | 0.51±0.14 | |||||
E5396 | 0.29±0.09 | b | 0.14±0.05 | b | 1.50±1.39 | 0.33±0.10 | b | ||
E5396/4 | 1.05±0.57 | ** | 0.37±0.16 | * | 1.64±1.61 | 0.72±0.61 | |||
R | 1.75±1.54 | ab | 1.14±0.45 | ab | 4.09±3.2 | 3.82±2.75 | a |
Values represent means and standard deviations calculated using Atub or PP2A gene as reference gene (n = 3–16). Statistical examination of data was performed using the independent-samples t test, Welch Two Sample t test or Wilcoxon test (with Bonferroni correction in the case of clonal differences). Different letters denote significant (P<0.05) difference between the clones. Stars denote significant (*P<0.05, **P<0.01, ***P<0.001) difference between transgenic line to non-transgenic clone.