Table 3. The relative expression of Bp4CL3 and Bp4CL4 genes in silver birch (Betula pendula) stems and leaves.
Gene | Stems | Leaves | |||||||
Clone/Line | Atub | PP2A | Atub | PP2A | |||||
Bp4CL3 | A | 1.27±0.21 | ad | 1.28±0.24 | a | 0.30±0.61 | a | 0.29±0.67 | a |
A1 | 0.85±0.25 | * | 0.48±0.15 | *** | 0.38±0.88 | 0.31±0.60 | |||
A5 | 1.28±0.18 | 0.78±0.4 | * | 0 | * | 0 | * | ||
E5382 | 0.52±0.05 | bc | 0.38±0.22 | b | 4.88±4.31 | bc | 0.79±0.24 | b | |
E5382/3 | 0.49±0.15 | 0.25±0.15 | 1.29±1.88 | ** | 0.97±1.17 | ||||
E5396 | 0.63±0.05 | b | 0.31±0.06 | b | 3.10±2.65 | bc | 0.91±0.70 | b | |
E5396/4 | 0.82±0.15 | * | 0.31±0.11 | 3.82±2.49 | 1.97±0.77 | ** | |||
R | 1.82±0.15 | cd | 0.56±0.2 | b | 1.75±1.02 | bc | 2.30±1.59 | c | |
Bp4CL4 | A | 0.88±0.22 | a | 0.88±0.16 | a | 5.15±3.83 | a | 6.01±4.00 | a |
A1 | 0.85±0.09 | 0.48±0.08 | *** | 3.36±2.79 | * | 2.40±1.44 | ** | ||
A5 | 1.17±0.54 | 0.71±0.19 | 2.84±2.40 | * | 1.40±1.05 | *** | |||
E5382 | 0.4±0.25 | ab | 0.33±0.28 | b | 0.43±0.40 | b | 0.15±0.31 | b | |
E5382/3 | 0.13±0.09 | 0.06±0.04 | 1.68±0.90 | *** | 1.92±1.09 | *** | |||
E5396 | 0.29±0.09 | b | 0.14±0.05 | b | 0.63±0.82 | bc | 0.23±0.38 | b | |
E5396/4 | 1.05±0.57 | ** | 0.37±0.16 | * | 1.81±1.53 | * | 0.93±0.51 | *** | |
R | 1.75±1.54 | ab | 1.14±0.45 | ab | 1.46±0.80 | c | 1.24±0.88 | c |
Values represent means and standard deviations calculated using Atub or PP2A gene as reference gene (n = 3–16). Statistical examination of data was performed using the independent-samples t test, Welch Two Sample t test or Wilcoxon test (with Bonferroni correction in the case of clonal differences). Different letters denote significant (P<0.05) difference between the clones. Stars denote significant (*P<0.05, **P<0.01, ***P<0.001) difference between transgenic line to non-transgenic clone.