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. 2014 Dec 11;9(12):e114434. doi: 10.1371/journal.pone.0114434

Table 3. The relative expression of Bp4CL3 and Bp4CL4 genes in silver birch (Betula pendula) stems and leaves.

Gene Stems Leaves
Clone/Line Atub PP2A Atub PP2A
Bp4CL3 A 1.27±0.21 ad 1.28±0.24 a 0.30±0.61 a 0.29±0.67 a
A1 0.85±0.25 * 0.48±0.15 *** 0.38±0.88 0.31±0.60
A5 1.28±0.18 0.78±0.4 * 0 * 0 *
E5382 0.52±0.05 bc 0.38±0.22 b 4.88±4.31 bc 0.79±0.24 b
E5382/3 0.49±0.15 0.25±0.15 1.29±1.88 ** 0.97±1.17
E5396 0.63±0.05 b 0.31±0.06 b 3.10±2.65 bc 0.91±0.70 b
E5396/4 0.82±0.15 * 0.31±0.11 3.82±2.49 1.97±0.77 **
R 1.82±0.15 cd 0.56±0.2 b 1.75±1.02 bc 2.30±1.59 c
Bp4CL4 A 0.88±0.22 a 0.88±0.16 a 5.15±3.83 a 6.01±4.00 a
A1 0.85±0.09 0.48±0.08 *** 3.36±2.79 * 2.40±1.44 **
A5 1.17±0.54 0.71±0.19 2.84±2.40 * 1.40±1.05 ***
E5382 0.4±0.25 ab 0.33±0.28 b 0.43±0.40 b 0.15±0.31 b
E5382/3 0.13±0.09 0.06±0.04 1.68±0.90 *** 1.92±1.09 ***
E5396 0.29±0.09 b 0.14±0.05 b 0.63±0.82 bc 0.23±0.38 b
E5396/4 1.05±0.57 ** 0.37±0.16 * 1.81±1.53 * 0.93±0.51 ***
R 1.75±1.54 ab 1.14±0.45 ab 1.46±0.80 c 1.24±0.88 c

Values represent means and standard deviations calculated using Atub or PP2A gene as reference gene (n = 3–16). Statistical examination of data was performed using the independent-samples t test, Welch Two Sample t test or Wilcoxon test (with Bonferroni correction in the case of clonal differences). Different letters denote significant (P<0.05) difference between the clones. Stars denote significant (*P<0.05, **P<0.01, ***P<0.001) difference between transgenic line to non-transgenic clone.