Table 6. Mitochondrial targeting prediction of fungal homologues of proteins in Table 5.
Gene name | Organism | Entry | Definition | MTS probability/cleavage site/AA sequence lengtha | Upstream MTS probability/cleavage site/AA sequence lengthb |
BPL1 | S. cerevisiae | YDL141W | Biotin:apoprotein ligase | 0.0101/NP/690 | 0.9329/51/760 |
K. lactis | KLLA0F05049g | hypothetical protein | 0.0477/NP/693 | 0.9870/30/732 | |
A. gossypii | AGOS_ADR085W | hypothetical protein | 0.0314/NP/679 | 0.6205/NP/720 | |
C. dubliniensis | CD36_35420 | biotin: apoprotein ligase | 0.0108/NP/665 | 0.6348/NP/689 | |
Z. rouxii | ZYRO0F05940g | hypothetical protein | 0.0219/NP/690 | 0.8872/30/722 | |
L.thermotolerans | KLTH0H02024g | biotin−protein ligase | 0.042/NP/660 | 0.4203/53/7110 | |
K. africana | KAFR_0B01050 | hypothetical protein | 0.0219/NP/690 | 0.8872/30/722 | |
FAA2 | S. cerevisiae | YER015W | medium-chain fatty acid-CoA ligase FAA2 | 0.1550/NP/744 | 0.8807/21/765 |
K. lactis | KLLA0B11572g | long-chain fatty acyl-CoA synthetase | 0.1983/NP/745 | 0.2603/NP/749 | |
C. glabrata | CAGL0H09460g | hypothetical protein | 0.3174/NP/741 | 0.4203/NP/752 | |
T. delbureckii | TDEL_0H02790 | hypothetical protein | 0.2323/NP/741 | 0.8615/NP/750 | |
SAM2 | S. cerevisiae | YDR502c | S-adenosylmethionine synthetase | 0.0373/NP/384 | 0.6377/NP/430 |
K. lactis | KLLA0C01782g | hypothetical protein | 0.0159/NP/384 | 0.1565/NP/392 | |
A. gossypii | AGOS_AFR692C | AFR692Cp | 0.3994/14/382 | 0.1639/NP/387 | |
Z. rouxii | ZYRO0F17248g | hypothetical protein | 0.0769/NP/382 | 0.3633/NP/412 | |
P. pastoris | PAS_chr3_0876 | S-adenosylmethionine synthetase | 0.0139/NP/384 | 0.5093/NP/423*1 | |
C. glabrata | CAGL0J08415g | hypothetical protein | 0.0634/15/383 | 0.2737/29/397*2 | |
C. glabrata | CAGL0B01122g | hypothetical protein | 0.0727/NP/382 | 0.0308/NP/394 | |
C. dubliniensis | CD36_10740 | S-adenosylmethionine synthetase, putative | 0.0280/NP/385 | 0.9093/58/447 |
: The whole amino acid sequence of each protein was analyzed with MitoProt II. b: The 5′ upstream region of the ORF of each protein was analyzed up to the first in-frame stop codon. *1The P. pastoris SAM homolog is preceded by a 39 aa sequence with high probability of being an MTS and starting with a bona fide ATG that apparently has been omitted in the annotation, probably because this sequence does not show homology with the S. cerevisiae SAM1 or SAM2 genes. *2 Translation from the first likely initiation codon (ATA, Ile) results in a probability of 0.4938.