Table 5. V3 amino acid sequences and predicted phenotypes of different HIV-1 isolates in Taiwan.
Isolate | Consensus V3 region amino acid sequences 11 1819 232425 CTRPNNNTRKSIHIGPGRAFYTTGEIIGDIRQAHC | Genetic subtype | Biotype | MT-2 assay | Predictions | ||
Sequencea | Geno2phenob | PSSMc | |||||
TW_D78 | CTRPGNNTRKSIRIGPGQTFYATGDIIGDIRQAHC | CRF07_BC | R5 | NSI | R5 | R5 | R5 |
TW_D83 | CTRPGNNTRKSIRIGPGQTFYATGDIIGDIRQAHC | CRF07_BC | R5 | NSI | R5 | R5 | R5 |
TW_D590 | CTRPGNNTRKSIRIGPGQTFYATGDIIGDIRQAHC | CRF07_BC | R5 | NSI | R5 | R5 | R5 |
TW_D592 | CTRPGNNTRKSIRIGPGQTFYATGDIIGDIRQAHC | CRF07_BC | R5 | NSI | R5 | R5 | R5 |
TW_D848 | CTRPGNNTRKSIRIGPGQTFYATGEIIGNIRQAHC | CRF07_BC | R5 | NSI | R5 | R5 | R5 |
TW_D854 | CTRPGNNTRKSIRIGPGQTFYATGDIIGDIRQAHC | CRF07_BC | R5 | NSI | R5 | R5 | R5 |
TW_D855 | CTRPGNNTRKSIRIGPGQTFYATGDIIGDIRQAHC | CRF07_BC | R5 | NSI | R5 | R5 | R5 |
TW_D40 | CTRPNNNTRRSIPIGPGRAFYTSE-IIGDIRKAHC | B | R5 | NSI | R5 | R5 | R5 |
TW_D257 | CTRPNNNTRKSISMGPGKAFFATGDIIGDIRAAYC | B | R5 | NSI | R5 | R5 | R5 |
TW_D573 | CTRPNNNTRKSIPIGPGRAFYTTN-IIGDIRKAHC | B | R5 | NSI | R5 | R5 | R5 |
A dash indicated a deletion or lack of an insertion.
The phenotype prediction based on 2 amino acid insertion/deletion between position 14 and 15, as well as variable amino acid positions (11, 18, 19, 23, 24 and 25) of V3 regions.
Geno2pheno (http://coreceptor.bioinf.mpi-inf.mpg.de/index.php), false-positive rate of 0.01.
Position-Specific Scoring Matrix (PSSM) (http://indra.mullins.microbiol.washington.edu/webpssm/).