Table 2. Antigenic reactivity of cervical T cell lines.

ID	Study Day	HSV-2 protein															Proteins + per cytobrush
		glycoprotein		immediate-early				capsid			tegument				other
		gD-2	gB-2	ICP0	ICP22	ICP27	ICP4	UL19	UL25	UL35	UL46	UL47	UL49	UL11	UL39	UL29
3	4/5/2010						+				+				+		3
	6/11/2010	+		+			+				+				+		5
	2/8/2011	+	+				+	+			+		+		+		7
4	4/20/2010										+		+		+		3
5	4/21/2010						+	+									2
	7/12/2010			+						+		+			+		4
6	4/26/2010					+		+									2
8	5/17/2010						+				+		+				3
10	6/1/2010		+							+			+				3
	7/1/2010			+			+			+			+		+		5
	5/10/2011		+										+				2
11	6/7/2010										+				+		2
	7/14/2010			+			+								+		4
13	8/4/2010						+	+			+					+	4
14	8/9/2010	+	+			+	+	+			+		+		+	+	9
15	7/1/2010		+						+				+				3
17	7/19/2010	+	+				+	+			+		+		+		8
	8/20/2010	+	+			+		+			+		+		+	+	9
	2/8/2011	+	+					+			+				+		5
18	7/20/2010	+			+		+	+				+	+	+		+	7
	8/19/2010	+		+									+	+	+		5
19	7/22/2010					+		+			+		+				4
20	7/2/2010		+	+			+								+		4
	8/23/2010			+			+								+		3
	2/15/2011				+										+		2
21	7/27/2010						+								+		2
23	9/2/2010	+													+		2
26	9/9/2010	+					+					+			+		4
28	8/12/2010		+														1
	9/14/2010	+	+	+			+			+					+		6
29	8/19/2010	+									+	+	+		+		5
	9/20/2010	+	+	+			+	+			+	+	+	+	+		10
	3/22/2011	+	+	+	+	+	+	+			+		+	+	+		11
#pos/subject (n=19)		8	8	8	4	5	14	9	1	3	9	4	10	2	14	4	4

Cervical T cell lines from HSV-2+ participants with positive HSV-2 specific lymphoproliferative responses were screened by IFN-γ ELISPOT using 34 pools of overlapping peptides representing 16 HSV-2 proteins that are classified as glycoproteins, immediate-early, capsid, tegument or other proteins. Positive IFN-γ ELISPOT responses to sub-pools are grouped together. +, positive IFN-γ ELISPOT response; shaded cells, T cells directed at the same HSV-2 protein detected in more than one cytobrush sample from the same participant.