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. 2014 Dec 11;9(12):e114899. doi: 10.1371/journal.pone.0114899

Figure 5. Effects of cyc, Clk, and tim dsRNA (dscyc, dsClk, and dstim) on mRNA levels of HR3 and E75 in firebrats.

Figure 5

(A) Dscyc, dsClk, and dstim abolished the rhythmic expression of HR3 with significant downregulation of mRNA levels. (B) RNAi of the three genes abolished the rhythm of E75. Dstim effectively downregulated E75 mRNA but dscyc and dsClk had no significant effects, except at ZT18 where a significant increase was observed. *P<0.05, **P<0.01, t test vs dsDsRed2. Data for dsDsRed2 firebrats are replotted from Fig. 2. For further explanation, see text and Fig. 2.