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. 2014 Dec 11;9(12):e114899. doi: 10.1371/journal.pone.0114899

Table 1. PCR primers used for PCR, quantitative RT-PCR, and dsRNA synthesis.

Genes Forward Reverse
Degenerate PCR
HR3 5′-ccagtcctccgtggtgaaYtaYcaRtg-3′ 5′-cggggaccatcttggcRaaYtcDat-3′
E75 5′-cgcatcctggccgcNatgcaRca-3′ 5′-acttcttgtggggcttgtaggYYtcRtccat-3′
Quantitative RT-PCR
HR3 5′-CGAGGTTCGGTTTCACAGAG-3′ 5′-GAGGAGGACGGTTGTTGTTG-3′
E75 5′-GAAATGCCCAGCTACACCAC-3′ 5′-GAACTCAACAACCCCACGAA-3′
Timeless 5′-TACAAGCCAGGTCCATCACA-3′ 5′-TCAAGCGTCAATTCAGCATC-3′
Clock 5′-ATCGCAAGGGTCTGGAAGTG-3′ 5′-GGAAAACTCGCCAAGACAGG-3′
Cycle 5′-CGTGTAATCTGTCGTGTTTGGTG-3′ 5′-GAATCGTCCGCCTTTCCTC-3′
rp49 5′-AGTCCGAAGGCGGTTTAAGG-3′ 5′-TACAGCGTGTGCGATCTCTG-3′
dsRNA synthesis
HR3#1 5′-TAATACGACTCACTATAGGGACTTCTCCTGCCTCGGGTAT-3′ 5′-TAATACGACTCACTATAGGGCAGTTTTAGCTCCGCCAAAC-3′
HR3#2 5′-TAATACGACTCACTATAGGGCGAAAATGGTACCTGGCTTT-3′ 5′-TAATACGACTCACTATAGGGTCGCTTGAATTTACCCAAGG-3′
E75#1 5′-TAATACGACTCACTATAGGGAGTCAAAGTCACCCCGAGAA-3′ 5′-TAATACGACTCACTATAGGGTCATTAGGCGAGTCCAGCTT-3′
E75#2 5′-TAATACGACTCACTATAGGGCAGAACCAATGGTGGTTTCC-3′ 5′-TAATACGACTCACTATAGGGGCCGCTGTTGTTGTAGAGGT-3′
timeless 5′-TAATACGACTCACTATAGGGTGCATTTGGTTGTGACTGCT-3′ 5′-TAATACGACTCACTATAGGGAGAGGCGTGTGCCTTGTACT-3′
Clock 5′-TAATACGACTCACTATAGGGACCACCAATCGAAAAATGGA-3′ 5′-TAATACGACTCACTATAGGGCCCAGTTCCCACGAAAACTA-3′
cycle 5′-TAATACGACTCACTATAGGGAGGGGCTGTTCATTCCTACA-3′ 5′-TAATACGACTCACTATAGGGCGCCCACGACTTCAAATAAC-3′
DsRed2 5′-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′ 5′-TAATACGACTCACTATAGGGCTACAGGAACAGGTGGTGGC-3′