Table 3. Effects of dsHR3, dsE75, and dsDsRed2 on the locomotor rhythm of the firebrat Thermobia domestica.
Treatment | n | No. of insects | Free-running period (mean ± SD) h | |
Rhythmic (%) | Arrhythmic (%) | |||
LD | ||||
HR3 dsRNA#1 | 6 | 6 (100) | 0 (0) | 24.0±1.6 |
E75 dsRNA#1 | 17 | 12 (71) | 5 (29) | 23.8±1.0 |
DsRed2 dsRNA | 20 | 18 (90) | 2 (20) | 23.9±0.3 |
DD | ||||
HR3 dsRNA#1 | 8 | 2 (25) | 6 (75)a | 24.2±0,6 |
HR3 dsRNA #2 | 4 | 0 (0) | 4(100)b | - |
E75 dsRNA #1 | 9 | 2 (22) | 7 (78)b | 24.5±0.4 |
E75 dsRNA #2 | 6 | 1 (17) | 5 (83)b | 24.3 |
DsRed2 dsRNA | 24 | 18 (75) | 6 (25) | 24.1±0.8 |
P<0.05, b P<0.001 vs DsRed2 dsRNA, Chi-square test; SD, standard deviation; LD, light–dark cycle; DD, constant darkness.