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. 2014 Dec 11;9(12):e114623. doi: 10.1371/journal.pone.0114623

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© 2014 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A): Total Nav1.8 protein. Upper: representative of Western blot bands; Lower: statistical analysis of Nav1.8 total protein expression. No significant difference is observed on the expression of Nav1.8 total protein among the three groups. P>0.05, two-way ANOVA, n = 4/group. (B): Nav1.8 membrane protein. Upper: representative of Western blot bands. Transferrin receptor (TfR) is used as internal control. Lower: statistical analysis of Nav1.8 membrane protein expression. Note that the expression of Nav1.8 on the cell membrane is increased significantly in the DRG neurons of MRMT-1-treated rats compared to PBS-treated rats. ^*** P<0.001, two-way ANOVA, n = 6/group. (C): Nav1.8 protein in the cytosol. Note that the expression of Nav1.8 in the cytosol is significantly decreased in the DRG neurons of MRMT-1-treated rats compared to PBS-treated rats. ^*** P<0.001, two-way ANOVA, n = 3/group.