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. 2014 Dec 11;9(12):e114546. doi: 10.1371/journal.pone.0114546

Figure 6. Optic nerve grading.

Figure 6

Optic nerve axon damage was assessed by masked, semi-quantitative grading [54], [55] of sections obtained 1 mm from the globe. (A) Results of optic nerve axon grading. (B-G) Representative examples of optic nerve cross-sections at 3-weeks stained with p-phenylenediamine. (B, C). Naïve normal (grade 1). (D) 2-month fellow control (grade 1.5). (E) 9-month fellow control (grade 1.5). (F) 2-month experimental (grade 4). (G) 9-month experimental (grade 5). Arrows point to unraveled myelin seen only in experimental nerves. Both experimental age-groups show severe axon damage relative to fellow control and naïve nerves. Fellow control nerves also show some focal degeneration/swelling relative to the naïve nerves but no unraveling of myelin. Error bars  =  SEM. * indicates p<0.05 (Dunn's post-hoc test). Abbreviations: EXP  =  experimental eyes, CTRL  =  fellow control eyes.