Skip to main content
. 2014 Dec 11;9(12):e115053. doi: 10.1371/journal.pone.0115053

Figure 4. Caspase-12 silencing altered NOD1, RIP2 and p-NF-κB protein expression in Leuk-1 cells.

Figure 4

A Immunofluorescence and confocal microscopy results showed that the transfection efficiency of Caspase-12 siRNAs was ∼95%. B FAM-labelled siRNAs indicated subcellular localization of cytoplasm, especially perinuclear area. C Western blot assay indicated protein level of Caspase-12 greatly decreased in Leuk-1 cells following RNA interference. D According to the image analysis result of immunoblot bands, compared with the NC group, the normalized protein level of Caspase-12 reduced remarkably in Caspase-12 siRNA-1 and -2 groups, especially for Caspase-12 siRNA-2. E Real-time PCR showed that the transfection of Caspase-12 siRNA-2 caused clear Caspase-12 silencing at mRNA level. F Western blot assay indicated Caspase-12 silencing altered NOD1, RIP2, and p-NF-κB protein expression. G Immunoblotting demonstrated that NOD1 expression significantly increased in Caspase-12-silenced cells compared with that in NC group. H Other than NOD1, Caspase-12 silencing remarkably reduced RIP2 level. I Alike to NOD1, p-NF-κB expression was up-regulated in Caspase-12-silenced cells. The immunoblot band density and mRNA data were expressed as means±SE (n = 3). Statistical significance: *P<0.05, **P<0.01, ***P<0.001 vs. scrambled siRNA-transfected cells.