Figure 2. GzmB^W cleaves Bid more efficiently and procaspases less efficiently than GzmB^P.

In vitro translated ³⁵S-labeled mouse procaspase 3, mouse procaspase 7, or mouse Bid were incubated with the indicated amounts of granzymes at 37°C for 30 min. Products were separated by 15% SDS-PAGE and visualized by fluorography.