Figure 8. GzmB^W/W CD8 T cells are unable to lyse MCMV infected cells.

(A) The numbers of CD8 and CD4 T cells localizing to the liver of B6 mice (black bar) or GzmB^w/w mice (white bar) after infection with MCMV Δm157 are shown. (B) At the indicated times post-infection, splenocytes were stained with anti-CD8, anti-TCRβ, and M45 tetramers. Representative FACS plots showing the percentage of M45-specific CD8 T cells are shown, and (C) the total numbers of M45-specific CD8 T cells are plotted. Data are pooled from 2 independent experiments, where n≥5. (D) Splenocytes were isolated from MCMV Δm157 infected B6 (black square) and GzmB^w/w mice (white circle), or from uninfected B6 mice (white square) and GzmB^w/w mice (black circle). Splenocytes were cultured with ⁵¹Cr-labeled M45 pulsed EL4 cells for 4 h and specific lysis determined. n = 6 for each data point. (E) CD8 T cells were purified from B6 mice (black square) and GzmB^w/w mice (open circle) and co-cultured with MCMV infected IC-21 macrophages for 18 h at the indicated E∶T ratios. n = 5 for each data point.