Table 1. GzmBW has a substrate preference similar to human GzmB.
Substrate | k cat(s−1) | K M (mM) | k cat/K M (M−1s−1) | |
Human GzmB | ||||
Abz-IEPD SESQK-dnp | (JNI-7) | 1.316±0.091 | 18.96±2.45 | 69 397±10 161 |
Abz-IEPD SGSQK-dnp | (JNI-9) | 5.106±0.270 | 22.06±2.67 | 231 449±30 585 |
Abz-LEYD LGALK-dnp | (JNI-13) | 0.035±0.002 | 122.90±6.83 | 285±20 |
Mouse GzmBP | ||||
Abz-IEPD SESQK-dnp | (JNI-7) | 0.049±0.004 | 30.88±2.48 | 1 575±172 |
Abz-IEPD SGSQK-dnp | (JNI-9) | 0.651±0.182 | 59.67±2.92 | 10 915±3101 |
Abz-LEYD LGALK-dnp | (JNI-13) | 2.510±0.160 | 23.48±2.68 | 106 948±18 094 |
Mouse GzmBW | ||||
Abz-IEPD SESQK-dnp | (JNI-7) | 6.14±0.54 | 30.32±5.60 | 202 364±17 877 |
Abz-IEPD SGSQK-dnp | (JNI-9) | 24.45±1.31 | 21.22±2.35 | 1 152 328±61 606 |
Abz-LEYD LGALK-dnp | (JNI-13) | 1.97±0.07 | 83.80±5.06 | 23 573±1 649 |