Figure 1. A. A schematic depiction of NoV RNA1 and its translation and replication products.

RNA is shown in green; hexagons indicate the cap structure. ORF A is translated from RNA1 and initiates replication of RNA1 and RNA3. ORF B1 is equivalent to the C-terminus of ORF A, while ORF B2 is frameshifted with respect to A and B1. The extreme C-terminal fragment of proteins A and B1 (A^C-TERM), which is mutated below (See Fig. 7), is shown in magenta. Also, the location of the GDD motif of the polymerase is shown; the GDD→GAA mutant is used as a non-replicating control throughout this work. B. Design of the constructs used to express NoV RNA1 and replicons. See text for detailed description. C. Northern analysis of plasmid transfection. Left lane: plasmid encoding wildtype (GDD) polymerase; right lane: plasmid encoding a control (GAA) polymerase. The background bands common to both lanes correspond to rRNAs.