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. 2014 Dec 11;10(12):e1004533. doi: 10.1371/journal.ppat.1004533

Figure 1. Schematic representation of study design and experimental procedures.

Figure 1

(A) Eight rhesus macaques were infected with SIVmac251 and CD8 T lymphocyte depleted on 6, 8 and 12 dpi. In all animals, CNS changes were monitored by 1H MRS prior to infection (−15, −9 dpi) and biweekly throughout infection (13, 26, 41, 48 dpi). Four macaques were treated with natalizumab weekly for three weeks (late; 28, 35, and 41 dpi) once significant neuronal injury was observed by 1H MRS. Two untreated and two natalizumab treated macaques received BrdU throughout the experiment (−9 dpi, 26 dpi, and 24 hours prior to sacrifice) to determine the total number of cells trafficking into tissues. Two untreated and two treated macaques received BrdU on 33 dpi and 24 hours prior to sacrifice to compare the number of cells trafficking into tissues with and without natalizumab. All macaques were sacrificed upon progression to AIDS between 49–62 dpi. (B) Nine rhesus macaques were infected with SIVmac251 (0 dpi) and CD8 T lymphocyte depleted on 6, 8, and 12 dpi. Six macaques received weekly natalizumab infusions beginning at the time of infection (0 dpi), and on 7 and 14 dpi (early). Animals received BrdU on 7 and 20 dpi to monitor the total number of cells trafficking into tissues and were all sacrificed at 22 dpi.