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. 2014 Oct 15;289(50):34490–34502. doi: 10.1074/jbc.M114.604132

FIGURE 2.

FIGURE 2.

Ube2j1−/− MEFs and organs express higher levels of specific ER dislocation components, and Ube2j1−/− MEFs are more susceptible to SV40 infection. A, immunoblots of several components of the ER quality machinery in two independent Ube2j1+/+ (WT) or Ube2j1−/− (KO) MEF lines, respectively, show increased levels for specific ER quality control components in Ube2j1−/− MEFs. B and C, protein levels in organ lysates from a Ube2j1−/− mouse and a wild type littermate. SI, small intestine; LI, large intestine; SG, salivary gland; SM, skeletal muscle; LN, lymph nodes; Epi, epididymis. D, transcript levels of select genes involved in the ER stress response and ER dislocation are unaltered in Ube2j1−/− MEFs. Shown are average reads per kilobase per million mapped reads (RPKM) values as determined by RNA-Seq from two independent MEF cell lines (passage 3) per genotype. E, wild type or Ube2j1−/− MEFs were infected with different MOIs of SV40. 24 h after infection the expression of the large T antigen was measured by FACS. Shown are mean and standard deviation values of relative percentages of infected cells of three independent experiments. **, p < 0.01; ***, p < 0.001, as determined by Student's t tests. F, binding of cholera toxin B subunit (FITC-CTxB) to wild type or Ube2j1−/− MEFs. Shown are mean MFI values and standard deviation of two wild type or two Ube2j1−/− MEF lines, respectively, from one representative experiment.