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. 2014 Oct 15;289(50):34490–34502. doi: 10.1074/jbc.M114.604132

FIGURE 3.

FIGURE 3.

Ube2j1-deficiency does not enhance induction of the UPR. A, representation of the reads measured for Xbp-1 by RNA-Seq. Shown are reads from unstimulated Ube2j1+/+ (WT) or Ube2j1−/− (KO) MEF lines (passage 3). The bottom panel depicts the intron/exon structure of Xbp-1. B, splicing of the Xbp1-mRNA after exposure of Ube2j1+/+ and Ube2j1−/− MEFs to tunicamycin as measured by RT-PCR. B, proteolytic processing of ATF6 after exposure of Ube2j1+/+ and Ube2j1−/− MEFs to tunicamycin measured by immunoblotting with an antibody directed to the N terminus of ATF6.