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. 2014 Oct 15;289(50):34490–34502. doi: 10.1074/jbc.M114.604132

FIGURE 5.

FIGURE 5.

Ube2j1-deficiency causes male sterility in mice. A, total number of sperm cells in the epididymis of Ube2j1+/+ (WT) or Ube2j1−/− (KO) mice. *: p < 0.05, as determined by unpaired t test. B, progressive motility of Ube2j1−/− sperm cells is impaired. Shown are average percentages of (black) immotile, (white) non-progressively motile, and (gray) progressively motile Ube2j1+/+ (WT) or Ube2j1−/− (KO) sperm cells (n≈200 per mouse, 3 mice per genotype). C, assessment of sperm cell morphology. Average percentages of (black) morphologically normal, (white) acephalic and (gray) morphologically abnormal sperm cells as observed in an ethanol-fixed sperm smear are shown (n≈200 per mouse, 3 mice per genotype). D, photographs of ethanol-fixed Ube2j1+/+ (WT) or Ube2j1−/− (KO) spermatozoa. Note the abnormal head morphology in the knock-out sperm. E, percentages of embryo stages observed in an IVF experiment 1, 2, and 3.5 days after fertilization. Spermatozoa from Ube2j1+/+ (WT) or Ube2j1−/− (KO) mice (3 mice per genotype) were incubated with wild type oocytes (∼200 oocytes per sperm of one male) and embryos at the indicated stages were counted.