TABLE 3.

Results from SPR measurements using purified proteins

Immobilized proteina	Protein partnerb	KDc
		μm
TUM1-Iso1	NFS1Δ1–55	1.63 ± 0.14
	NFS1Δ1–55·ISD11	0.18 ± 0.08
	BSA	NDd
	E. coli IscS	ND
TUM1-Iso2	NFS1Δ1–55	1.11 ± 0.21
	NFS1Δ1–55·ISD11	0.26 ± 0.10
	BSA	ND
	E. coli IscS	ND
NFS1Δ1–55	TUM1-Iso1	4.06 ± 1.19
	TUM1-Iso2	2.45 ± 0.55
	BSA	ND
	E. coli IscS	ND
NFS1Δ1–55·ISD11	TUM1-Iso1	2.92 ± 0.76
	TUM1-Iso2	2.91 ± 1.22
	BSA	ND
	E. coli IscS	ND
MOCS3	TUM1-Iso1	3.07 ± 0.08
	TUM1-Iso2	3.58 ± 0.55
	BSA	ND
	E. coli IscS	ND
MOCS3-RLD	TUM1-Iso1	2.61 ± 0.33
	TUM1-Iso2	3.19 ± 1.8
	BSA	ND
	E. coli IscS	ND

^a Proteins were immobilized via amine coupling.

^b Proteins were injected by application of the KINJECT protocol. Concentrations were in the range of 0.3–20 μm. Regeneration of the flow cell was achieved by injection of 20 mm HCl.

^c K_D values and standard deviations were obtained by global fitting to a 1:1 binding model from at least two independent measurements.

^d ND, no binding detectable.