FIGURE 2.

GAL-A activity assays for the galB-D mutants and gene complementation studies. GAL-A activity was assessed by using C. neoformans as the indicator organism. The culture supernatant was extracted with ethyl acetate (A and C–E), and the mycelial paste was extracted with methanol (B). The assay results of WT, ΔgalB::aacIV (ΔgalB), ΔgalC::aacIV (ΔgalC), and ΔgalD::aacIV (ΔgalD) are shown in A and B, where the amounts of mutant extract applied were four times that of WT. The in trans gene complementation results of ΔgalB::aacIV (C), ΔgalC::aacIV (D), and ΔgalD::aacIV (E) are shown alongside the descriptions of the complementation constructs (F). The lane identities for C–E are: WT (1), each mutant (2), each mutant with ermEp-galABCDE (3), each mutant with the target gene under ermEp control (4), and each mutant with the vector control (5). The position of GAL-A is indicated by arrows and lines.