FIGURE 1.

Tim23^IMS and mitochondria-like membranes. A, amino acid sequence of Tim23^IMS (residues 1–96). Two additional residues (Gly-Ser) are present due to His tag removal by tobacco etch virus nuclear inclusion a endopeptidase cleavage. The two membrane-binding regions are highlighted in red. B, electron micrograph of liposomes mimicking the phospholipid composition of the OMM. C, superposition of ¹H-¹⁵N heteronuclear single quantum coherence (HSQC) spectra of 0.05 mm Tim23^IMS without (black) and with (red) the addition of a 3-fold molar excess of total lipids in OMM-like liposomes. Selected resonance assignments are indicated.