FIGURE 5.
Effects of spermine and ion channel blockers on duodenal Isc and DBS in KCNQ1 KO and WT mice. A, spermine-stimulated net peak DBS in the presence or the absence of chromanol 293B (10 μm), clotrimazole (30 μm), or 2-APB (100 μm) in wild-type mice. B, comparison of spermine-stimulated net peak DBS in KCNQ1 knockout and wild-type mice. C, comparison of the time course of duodenal Isc in KCNQ1 knockout and wild-type mice after addition of spermine to Ussing chambers as indicated by the arrow. Spermine was used at 1 mm in A–C. Values are expressed as mean ± S.E. for six experiments. **, p < 0.01 versus the control (spermine-stimulated net peak DBS in wild-type mice).